Composite

Part:BBa_K2128102:Design

Designed by: Kathryn Flanagan   Group: iGEM16_Genspace   (2016-10-16)


Strong promoter/RBS for HDLEA3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have attempted to maximize expression of HDLEA3.


Source

The coding sequence of this composite part is a member of a class of intrinsically disordered proteins called the Late Embryonic Abundant (LEA) proteins that become ordered when desiccated, thereby stabilizing DNA and protein cell components. It was taken from the genome of H. Dujardini and optimized by the IDT Codon Optimization Tool for E. Coli K12.

References